CLINICAL DYNAMICS OF VARICOSE DISEASE IN PATIENTS WITH HIGH DEGREE OF VENOUS REFLUX DURING CONSERVATIVE TREATMENT AND AFTER SURGERY: A 7-YEAR FOLLOWUP
Lurie F, Makarova NP.
Intl J Angiol 1998; 7:234-37


COMMENTARY BY:
Michel Perrin, MD
Lyon, France

This study included 190 limbs (183 patients). All patients were accurately described clinically and hemodynamically and all were grade 2 or 3 according to the Porter Classification of Chronic Venous Insufficiency in 1988.1 More than half of the patients (99) had had an active ulcer for more than six months.

Hemodynamics: All of the patients had a greater saphenous vein incompetence with reflux at the termination of the superficial femoral vein. All of the reflux was significant in duration (more than 1.7 second) with velocity less than 30 mm per second. The reflux index was 0.05. Concomitant reflux in the deep system was limited to grade 1 and 2 in almost all of cases according to the classification by Kistner.2 Only 3% were classified as grade 3 and only 2% were grade 4. Incompetent leg perforators were identified. Unfortunately, the frequency of perforators is given only in those who were treated by surgery (41.7%; 53/127).

The aim of this study was to determine the value and efficacy of three different treatment methods: Group 1, compression treatment alone (68 limbs), group 2, lesser saphenous vein stripping with or without perforator ligation (75 limbs), and group 3, valve repair with lesser saphenous vein stripping with or without perforator ligation (52 limbs). Duration of followup was longer than five years in 91% in group 1, 75% in group 2, and 76% in group 3. Results were assessed according to the classification mentioned above.

There was no statistical difference in results between the three groups if the prior history of the disease did not exceed five years. In the groups with a longer prior history, 27.7% patients improved in group 1, 46.8% in group 2, and 60.3% in group 3. In terms of recurrent varicose veins, recurrence was higher in group 2 than in group 3 (17.3% versus 7.7%). Measurement of reflux index at the termination of the superficial femoral vein was significantly improved only in group 3.

Correlation between hemodynamic and clinical results (recurrent ulcer) was good. Recurrent ulcer was noted in 73.7% of patients when deep vein reflux was not improved, 13.7% when stable, and 0% when deep reflux was ablated by the intervention.

COMMENTARY

Some of the results in the current study are consistent with previously published studies. When deep venous insufficiency is not treated, the risk of recurrent varicose veins is increased.3,4 Good correlation between clinical and hemodynamic results was found after valve repair (group 3). There was no recurrent ulcer (0/38) when femoral vein reflux was improved. There was a 7.8% recurrence (4/51) when femoral vein reflux was stable and a 73.7% recurrence (28/38) when this reflux was worsened. The correlations found in this study are greater than in previously published articles.5-7

On the other hand, some results are not in accordance with previous reports. The number of patients with an active ulcer of more than six months' duration was particularly great in this cohort with lesser saphenous vein insufficiency and moderate reflux in the femoropopliteal axis. When grade 1 or grade 2 reflux is present in the deep system, lesser saphenous vein stripping does correct reflux in the deep system.8-10 This improvement was not pointed out in the present study. ivdfper


REFERENCES

1. Porter JM, Rutherford RB, Clagett CP, et al. Reporting standards in venous disease. J Vasc Surg 1988; 8:172-181.

2. Kistner RL. Classification of chronic venous disease. Vascular Surgery 1997; 3:217-18.

3. Almgren B, Eriksson I. Valvular incompetence in superficial, deep, and perforator veins of limbs with varicose veins. Acta Chir Scand 1990; 1:69-74.

4. Perrin M, Bayon JM, Hiltbrand B, Nicolini P. Deep venous insufficiency and recurrent varicose veins after surgery of superficial venous insufficiency. J Maladies Vasculaires 1997; 22:343-47.

5. Kistner RL. Definitive diagnosis and definitive treatment in chronic venous disease: A concept whose time has come. J Vasc Surg 1996; 24:703-10.

6. Perrin M, Nicolini P. Reconstructive surgery in chronic venous disease of the lower limbs. Rev Med Suisse Romande 1997; 117:113-16.

7. Sottiurai VS. Surgical correction of recurrent venous ulcer. J Cardiovasc Surg (Torino) 1991; 332:104-09.

8. Walsh JC, Bergan JJ, Beeman S, Comer TP. Femoral venous reflux abolished by greater saphenous vein stripping. Ann Vasc Surg 1994; 8:566-70.

9. Padberg FT, Pappas PJ, Araki CT, Back IL, Hobson RW. Hemodynamic and clinical improvement after superficial vein ablation in primary combined venous insufficiency with ulceration. J Vasc Surg 1996; 24:711-18.

10. Sales CM, Bilof ML, Petrillo KA, Luka NL. Correction of lower extremity deep venous incompetence by ablation of superficial varicosities. Ann Vasc Surg 1996; 10:186-189.






HAEMODYNAMIC PATHOPHYSIOLOGY OF VENOUS INSUFFICIENCY OF THE LOWER LIMBS
Franceschi C.
ActualitÈs Vasculaires Int 1997; 47:17-27


ABSTRACT AND COMMENTARY BY:
Attilio Cavezzi, MD
San Benedetto del Tronto (AP), Italy

Dr. Franceschi has summarized his thoughts on the pathophysiology of venous insufficiency in a lengthy article. The original may of great interest to readers of the Venous Digest .

Dr. Franceschi feels that lower extremity venous dysfunction is mainly caused by anatomic or functional blockage. Actual blockage may be caused by deep venous thrombosis, obliteration of vessels by sclerotherapy, or extrinsic compression such as in the Cockett syndrome. Functional obstruction is exemplified by venous drainage mechanism dysfunction. This includes valvular incompetence or muscle pump failure. Valve incompetence proximal to the muscle pump allows normal systolic blood flow but produces diastolic reflux. Valve incompetence distal to the muscle pump may produce bidirectional flow without venous disease if the muscle pump is efficient. Venous shunts react differently, depending on posture. For example, during ambulation, venous flow is toward the heart during systole and back into the deep veins through the incompetent saphenous vein during diastole. This may exhaust the muscle valve pump and dilate superficial incompetent veins. Each of the venous shunts has two pathways, one deep and one superficial. These are interconnected. Segmental deep reflux may occur near entry of incompetent superficial veins such as common femoral vein reflux in cases of incompetence of the saphenofemoral junction or popliteal reflux above an incompetent saphenopopliteal junction.

The concept of reentry of blood from varicose veins into the deep venous system is of great importance. The normal function of perforating veins is to aspirate superficial blood into the deep venous system. Pathologic reflux, that is diastolic flow from deep to superficial veins, occurs in perforating veins proximal to a normally functioning venous pump. In cases of primary varicose veins, many of the perforating veins perform a predominant aspirating function even if dilated. This is also true when the perforating vein is under a trophic lesion caused by primary, not postthrombotic disease. Even the smallest varicosities and telangiectasias are mainly a sign of incorrect drainage of blood in major veins. They are either a sign of blocked drainage of superficial subcutaneous venules or an overloading of venous capillaries.

Dr. Franceschi goes on to say that large varicose veins do not give rise to dystrophic changes such as ulceration if there is good reentry into the deep venous system at some distance from the perimalleolar region where there is an abnormal lack of normal venous drainage and a maximum gravity-induced overload. He further stresses the importance of avoiding the elimination of draining pathways, whether are diseased or normal. He feels it is necessary to disconnect pathologic veins but preserve drainage pathways and direct flow towards more efficient reentry perforators.

COMMENTARY

Commenting on an article like this is difficult, especially for one who does not totally agree with Dr. Franceschi's CHIVA technique. On the other hand, this reviewer appreciates most of the CHIVA diagnostic approaches and premises.

The hemodynamic models which have been proposed by careful research and the use of color Doppler has helped us to understand why saphenous disease is mainly a result of reflux. One thing should be said regarding CHIVA treatment. If the function of the muscle valve pump is important in the pathogenesis of venous insufficiency, then the CHIVA method is clearly muscle valve pump dependent. The question arises whether in time the muscle valve pump will be compromised, thus threatening the hemodynamic correction achieved by the CHIVA operation.

One interesting concept supported by color duplex scanning is that deep venous incompetence may be caused by superficial venous incompetence. The common femoral and popliteal veins are affected by reflux in the adjacent saphenous junctions. Saphenous incompetence is capable of overloading the deep venous system with its own private recirculation.

Regarding the hemodynamics of perforating veins, it does appear that venous trophic disorders are a sign of superficial microvascular hydrostatic hypertension.

The conclusions reached in this article are characterized by recognition of the hereditary/constitutional nature of venous insufficiency and therefore of our own ignorance in the origins of varicose veins. The article highlights the importance of hemodynamic investigation before any treatment. It may be the role of the echo-color Doppler to bring specialists together to share and perfect their experiences.

Many more points are made by Dr. Franceschi but space limitations have allowed only a discussion of the principal concepts. vdbercav






PERICAPILLARY FIBRIN CUFFS IN VENOUS DISEASE: A REAPPRAISAL
Van de Scheur M, Falanga M
Dermatol Surg 1997; 23:955-59


ABSTRACT AND COMMENTARY BY:
H.A.M. Neumann, MD
Department of Dermatology
University Hospital Maastricht
Maastrict, The Netherlands

In this review, the authors focus on the complex pathogenesis of venous disease with special attention to the role of fibrin cuffs. Browse and Burnand postulated that venous ulceration is due to pericapillary fibrin cuffs which act as barriers to the diffusion of oxygen and nutrients. The barrier function of these cuffs is highly debated since the cuffs tend to be discontinuous around dermal capillaries and the extent of fibrin deposition may not be related to the relative degree of venous insufficiency. The fibrinogen molecule has a trinodular structure. Three types of chains exist. Higher order of cross-linked chains occurs through the formation of gamma chains. Perhaps this structure contributes to the resistance of fibrinolysis of the fibrin cuffs.

It has been suggested that patients with venous disease lack an "escape route" from the conversion of fibrinogen to cross-linked fibrin. Fibrin may contribute to the inflammatory process in lipodermatosclerosis. In addition to the fibrin cuff hypothesis, white cell trapping is gaining importance in the pathogenesis of venous disease.

Recently, Falanga and Eaglstein proposed an alternative hypothesis. They hypothesized that not only fibrin but macromolecules leak into the dermis and "trap" growth factors which renders them unavailable for the homeostasis of tissue integrity and the repair process. Fibrin itself may be the most important factor but it acts as a marker for decompensation of the venous system and especially for the larger problem of macromolecular leakage.

COMMENTARY

This review summarizes many of the ideas concerning the pathogenesis of venous disease. The fibrin cuff theory was one of the first recent hypotheses to be published (1982).1 Important to this is the knowledge that reflux in the veins results in alterations of the microcirculation. All skin changes, including leg ulcers, are the result of failing microcirculation. Particularly relevant to this discussion is microangiopathy, a prominent feature in chronic venous insufficiency (CVI).2 Bollinger, et al. combined the techniques of intracavital capillaroscopy and fluorescein angiography with videodensitometry.3 The results are suggestive of a diffusion-limited transport in those areas of the skin which have a decreased capillary density. Microthrombosis will occur, probably in relation to white cell trapping, which results in diminishing capillary density.

In the fibrin cuff therapy, it was proposed that the high venous pressure in chronic venous insufficiency caused leakage of large molecules through capillaries into the pericapillary fibrin cuff. It has been shown that the cuff consists of fibrin and not fibrinogen.4 This cuff should act as an oxygen-diffusion barrier. In addition, a significant increase in the collage IV layer in or around the capillaries has been found. Over time, however, significant doubts arose about the role of fibrin as a diffusion barrier.5 Neumann, et al. suggested that fibrin may function as a matrix for the formation of collagen.6

In a prospective, comparative, human model study, 16 patients with chronic venous insufficiency, six patients with porphyria cutanea tarda without venous disease, four with ecthyma ulcers, and ten healthy volunteers were examined. Transcutaneous oxygen readings were significantly lower in venous patients than in all other groups. Fibrin cuffs were found in 8 of 16 venous patients, in all patients with porphyria cutanea tarda, and in three of four patients with ecthyma ulcers. Based on these results, it was concluded that fibrin cuffs alone do not act as a barrier for oxygen transport.7 Fibrin deposits are more an indicator of microcirculatory disturbances than as the cause of low TcPO2.

A human model mimicking venous insufficiency in patients with Klinefelter syndrome was described. These patients lacked objective hemodynamic signs of chronic venous insufficiency but all had skin alterations typical of CVI such as lipodermatosclerosis and atrophie blanche. Plasminogen activator inhibitor 1 (PAI-1) was significantly increased in these patients.8 As fibrinolysis is disturbed in tissue in CVI, it is very possible that white cell trapping combined with local disturbances in fibrinolysis is the final cause for the most significant and serious skin changes noted in chronic venous insufficiency: lipodermatosclerosis, atrophie blanche, and leg ulceration.

The final mechanism leading to non-healing venous leg ulcers is still unknown. We can only hypothesize, on information from the literature that dilated capillaries develop under the high venous pressure in venous disease. This ambulant high venous pressure (venous hypertension) leads to low flow. White cell trapping will occur as non-immunological binding to the capillary endothelium. White cell trapping causes occlusions in the microcirculation which leads to microthrombosis. As the capillaries occlude, the capillary density diminishes. This leads to low tissue oxygen levels.

Leakage of macromolecules leads to cuffs with fibrin as a marker but also other macroglobulins such as a-2 macroglobulin are a part of these cuffs. a-2 macroglobulin binds growth factors such as TGF-þ. Therefore, fibrin cuffs, which are only the indicators of a seriously disturbed microcirculation, attracted our attention to the pathway of low tissue oxygen tension and also to trapping of growth factors. This could explain why chronic venous insufficiency leads to leg ulcers and why they do not heal spontaneously. More studies are needed concerning these fascinating fibrin cuffs. vdneu034


REFERENCES

1. Browse NL, Burnand KG. The cause of venous ulceration. Lancet 1982; 2:243-45.

2. Leu AJ, Yanar A, Pfister G, et al. Mikroangiopathie bei chronischer ven–serum insuffizienz. Dtsch Med Wschr 1991; 116:447-53.

3. Bollinger A, J”ger K, Geser A et al. Transcapillary and interstitial diffusion of Na-fluorescein in chronic venous insufficiency with atrophy. Int J Microcirc Clin Exp 1982; 1:5-17.

4. Brakman M, Faber WR, Kerckhaert JAM et al. Immunofluorescence studies of atrophie blanche with antibodies against fibrinogen, fibrin, plasminogen activator inhibitor, factor VIII-related antigen, and collagen type IV. VASA 1992; 21:143-48.

5. Michel CC. Oxygen diffusion in edematous tissue and through pericapillary cuffs. Phlebology 1990; 5:223-30.

6. Neumann HAM, van den Broeck MJTB. Increased collagen IV layer in the basal membrane area of the capillaries in severe chronic venous insufficiency. VASA 1991; 20:26-29.

7. Neumann HAM, van den Broek MJTB, Boersma IH, Veraart JCJM. Transcutaneous oxygen tension in patients with and without pericapillary fibrin cuffs in chronic venous insufficiency, porphyria cutanea tarda, and non-venous leg ulcers. VASA 1996; 25:127-33.

8. Veraart JCJM, Hamulyak K, Neumann HAM, Engelen J. Increased plasma activity of plasminogen activator inhibitor 1 (PAI-1) in two patients with Klinefelter syndrome complicated by leg ulcers. Br J Dermatol 1994; 130:641-44.






THANKS TO THE 1998 CONTRIBUTORS

In 1998, the Venous Digest continued its commitment to improve the treatment of venous disease. Our success is dependent upon the excellent abstracting of articles and commentary provided by the following individuals. This is truly an international Who's Who of physicians interested in diagnosis and management of venous disorders. The Editorial Office extends many thanks to them for their good work. vd.contr

Allegra, Claudio, Rome, Italy
Andreozzi, Giuseppe, Padua, Italy
Ascher, Enrico, Brooklyn, New York

Ballard, Jeffrey, Loma Linda, California
Bass, Arie, Zerifin, Israel
Bergqvist, David, Uppsala, Sweden
Bl”ttler, Werner, Z¸rich, Switzerland
Bradbury, Andrew, Edinburgh, Scotland
Bundens, Warner, San Diego, California
Burnand, Kevin, London, United Kingdom

Cairols, Marc, Barcelona, Spain
Caprini, Joseph, Evanston, Illinois
Charlesworth, Peter, Auckland, New Zealand
Conrad, Peter, Sydney, Australia
Cordts, Paul, Tripler, Hawaii

D'Addato, Massimo, Bologna, Italy
DeBarros Jr., Newton, Sao Paulo, Brazil
DePalma, Ralph, Reno, Nevada

Eklof, Bo, Honolulu, Hawaii

Fagrell, Bengt, Stockholm, Sweden
Fischer, Reinhard, St. Gallen, Switzerland
Fronek, Arnost, La Jolla, California

Goldman, Mitchel, La Jolla, California
Gradman, Wayne, Los Angeles, California
Green, David, Bethesda, Maryland
Green, David, Chicago, Illinois
Guex, J.-Jerome, Nice, France

Hingorani, Anil, Brooklyn, New York
Hoffmann, Ulrich, Z¸rich, Switzerland
Hoshino, Shunichi, Fukushima, Japan
Hunter, Glenn, Galveston, Texas

Kerstein, Morris, Philadelphia, Pennsylvania
Killewich, Lois, Baltimore, Maryland

Labropoulos, Nicos, Maywood, Illinois
Lalka, Stephen, Indianapolis, Indiana
Linde, N., St. Gallen, Switzerland

Mackiewicz, Zygmunt, Bydgoszcz, Poland
McIntyre, W. Burley, Loma Linda, California
Murray, Jay, San Diego, California
Myers, Kenneth, Richmond, Australia

Nachbur, Bernard, Berne, Switzerland
Norgren, Lars, Lund, Sweden

Olivencia, Jose, Des Moines, Iowa
Ouvry, Paul, Toulouse, France
Ouvry, Pierre, Toulouse, France

Padberg, Frank, Jr., East Orange, New Jersey
Partsch, Hugo, Vienna, Austria
Perrin, Michel, Decines, France
Pfeifer, John, Troy, Michigan

Rooke, Thomas, Rochester, Minnesota
Ruckley, C. Vaughan, Edinburgh, Scotland

Sales, Clifford, Belleville, New Jersey
Sanders, Richard, Denver, Colorado
Sapio, Nicola, Berne, Switzerland
Schmid-Sch–nbein, Geert, La Jolla, California
Schoevaerdts, Jean-Claude, Godinne, Belgium
Sobel, Michael, Richmond, Virginia
Somjen, George, Melbourne, Australia
Sottiurai, Vikrum, New Orleans, Louisiana
Stemmer, Robert, Illkirch, France
Struckmann, Jan, Charlottenlund, Denmark
Sykes, Mellick, San Antonio, Texas

Takase, Shinya, Fukushima, Japan

Vandendriesse, Marianne, Gent, Belgium
Villavicencio, J. Leonel, Bethesda, Maryland

Widmer, Matthias, Berne, Switzerland






A UNIFYING CONCEPT OF PRIMARY VENOUS INSUFFICIENCY
Bergan JJ
Dermatol Surg 1998; 24:425-28


ABSTRACT AND COMMENTARY BY:
Lois Killewich, MD
Assistant Professor of Surgery
University of Maryland Medical Center
Baltimore, Maryland

Dr. Bergan has written a concise, useful monograph on primary venous insufficiency, a disease process in which valvular incompetence develops in lower extremity veins without an antecedent episode of deep venous thrombosis. Valvular incompetence produces incomplete emptying of lower extremity veins with resultant dilatation and varicosity formation. Dr. Bergan begins by establishing the concept that varicose veins, reticular varicosities, and telangiectasias all represent different manifestations of this disease. He then discusses its underlying etiology, indications for intervention, and treatment options.

The development of primary venous insufficiency is influenced by heredity, hormones (particularly high progesterone levels), gravitational forces, and the calf muscle pump. This explains why varicosities develop during pregnancy and why deep venous incompetence can be translated into superficial and perforator incompetence through the pumping action of the calf muscles. The indications for intervention include pain, heaviness, fatigue, bleeding, thrombophlebitis, and ulceration. Ambulatory phlebectomy aimed at correcting the abnormal hydrostatic and hemodynamic forces remains the mainstay of surgical therapy.

COMMENTARY

Several of the concepts discussed in this paper deserve attention. The importance of the calf muscle pump in promoting the development of varicosities cannot be underestimated. When deep veins become incompetent, the volume of blood within their lumens increases. This results in increased pressure in the superficial veins which dilate, become incompetent, and increase pressure in the superficial veins. Since no further outlet for the increased volume and pressure exists, these vessels not only become incompetent but also dilate and become varicose. By increasing pressure in the deep and perforating veins each time contraction occurs, the calf muscle pump promotes this cascade. Interruption of the pressure (i.e., correcting incompetence in deep veins) can restore competence to superficial veins and provide at least partial relief.

Furthermore, physicians need to understand the relationship between symptoms such as leg aching and the various types of varicosities. Therapy cannot be initiated if the physician does not make this connection. Dr. Bergan makes the point that "...neither the patient nor the physician may understand that these symptoms [of venous insufficiency] arise from telangiectatic blemishes just as easily as from venous varicosities." He states further that "...85% [of these patients] will be relieved of them by appropriate therapy." Primary care physicians, surgeons, and dermatologists should be aware of these relationships since they are most likely to conduct the initial evaluation.

A final point for those of us who treat venous insufficiency surgically is the importance of assessing the saphenofemoral and saphenopopliteal junction and performing excision (rather than simple ligation) if either are found to be incompetent. Dr. Bergan cites many studies which demonstrate a high rate of recurrence if saphenous incompetence is ignored when ambulatory phlebectomy is performed. He also reminds us that it is unnecessary to remove the saphenous vein below the knee and that this results in a high incidence of saphenous nerve injury.

This paper provides useful information for the primary care physician and the practicing dermatologist or vascular surgeon. For those of us whose classical education is incomplete, Dr. Bergan could assist us by providing a definition of "atrophie blanche." vdkil288






Venous Disorders of the Legs: Principles & Practice
L. L. Tretbar
Springer-Verlag, London, 1998
139 pages with color plates



It is a refreshingly welcome experience to see a single-authored medical textbook. In this case, the author's personality comes through directly. His approach is straightforward and, as he says in the preface, he "speaks in ordinary English." The author himself is quite authoritative. He has a 30-year experience in America after one year spent in the Middlesex University Hospital in London. The Hospital is now the site of the most elegant clinical and basic science research on venous disorders.

Dr. Tretbar's personality also comes through in his execution of many of the drawings in the book and, as it turns out, he is a good artist as well as a good surgeon. As straightforward as the author himself is the organization of the volume. It contains sections on anatomy of leg veins, venous function, modern evaluation, and venous testing as well as sections on therapy. This reviewer found the history of medical and surgical treatments of varicose veins to be amusing and enlightening. Therapy by sclerotherapy as well as surgery is well covered and there are separate chapters on complications of chronic venous insufficiency and venous thromboembolic disease. It is important that Tretbar emphasizes that what used to be called the postphlebitic leg is now referred to as chronic venous insufficiency which implies that there is surgical treatment available.

Tretbar has been concerned mainly with treatment of varicose veins and it is in these sections that his work is most valuable. This is in contrast with the chapter on venous thromboembolic disease which, in other texts, occupies much more space and emphasis. It is junior surgeons often assigned the treatment of patients with venous disorders. As they are assigned responsibility, this book can be recommended to them. It is here that the anatomy and pathophysiology are well explained. Treatment based on that knowledge is also explained thoroughly.

Thus, this volume provides an introduction to the diagnosis and treatment of the most common venous disorders and hopefully will stimulate younger surgeons to accept the veins as part of their practice of vascular surgery. This book is recommended for the use of general surgeons, dermatologists interested in venous disease, and vascular surgeons who, in their fellowship, are interested in expanding their work to the other side of the vascular system. It is an attactive, well-designed, well-executed volume which fulfills its promise of describing principles and practice. book-015






A NOVEL CULTURING AND GRAFTING SYSTEM FOR THE TREATMENT OF LEG ULCERS
Villeneuve P, Hafner J, Prenosil JB, Elsner P, Burg G.
Br J Dermatol 1998; 138:849-51


COMMENTARY BY:
Prof. Ricardo Gesto
Madrid, Spain

Autologous tissue cultures and allogenic keratinocytes have been used for years to cover chronic skin defects of venous and diabetic foot ulcers. The clinical results of these studies have been heterogeneous. It is difficult to establish the usefulness of a technique with no data from controlled trials. The objective of this project was to develop an efficient system of culturing and grafting that was more rapid and easier to achieve than that described by Green et al. (Proc Nat Acad Sci USA 1979; 76:5665-68).

The culture system is a combination of two commercially available products. A Petriperm culture vessel was aseptically placed in a Petri dish. The bottom of the Petriperm disk was made of a fine Teflon film. This film was nonreactive, non-adhesive, flexible, permeable to gas, and transparent. The human epidermal cells were separated, processed, and cultured as described by Green with some modifications.The technique was used in a 61-year-old female who had suffered a superficial trauma which left her with a wound on the anterior surface of the right tibia. The ankle/arm index and oscillogram were normal and there were no clinical signs of venous disease. The Doppler did not show reflux of the deep and superficial venous systems. Local dressings and skin grafts had failed. The wound was finally treated with four epidermal grafts over a 12-week period using this method, showing good progress and eventual closure of the wound.

Culture of epidermal grafts requires trained personnel, an adequate medium, and a constant supply of fibroblasts. This method allows grafts to be obtained with less effort at a reasonable cost and could be used in other skin alterations such as vitiligo.

COMMENTARY

The authors describe obtaining skin graft cultures, modifying Green's method. This appears to be simpler with an adequate cost/benefit balance. As stated in the article, the usefulness of a technology must be confirmed through data based on controlled clinical trials. In this case, the clinical experience is based on a single case in a patient with no vascular disease and in whom a good result was obtained after other treatment forms had failed. The method needs to be compared in prospective clinical trials in order to be validated.

In general, patients with chronic ulcers of the lower extremities suffer from vascular disease responsible for the lesion. Therefore, it is imperative that treatment of these ulcers includes treatment of the underlying disease. Subsequently, treatment of the ulcer with the usual means (topical treatment or free skin grafts over large defects) offers no particular problems.

In any case, the new method seems to be a valid alternative treatment for chronic ulcers as it permits treatment of wounds with a variety of cell types simultaneously. However, this new method needs to be validated by comparative trials with other therapy. ivdfges






MINI ABSTRACTS
John J. Bergan, M.D.
Items of Interest Which Have Crossed the Editor's Desk
(Provided for reference purposes and general interest)



D-dimer Testing as an Adjunct to Ultrasonography in Patients with Clinically Suspected Deep Vein Thrombosis: Prospective Cohort Study
Bernardi E, Prandoni P, Lensing AWA, et al.
Br Med J 1998; 317:1037-40

A total of 946 patients with clinically suspected deep vein thrombosis were tested. The use of ultrasound and D-dimer testing allowed treatment decisions to be made which reduced the number of repeat ultrasound examinations and in 598 patients receiving testing who did not receive anticoagulation, only one thromboembolic episode occurred.